According to Transport for London (TfL), Uber will not be granted a new licence to operate in London.
The regulator said the taxi app was not “fit and proper” as a licence holder, despite having made a number of positive changes to its operations.
Uber originally lost its licence in 2017 due to safety concerns but was granted a 15-month extension. The firm says it will appeal against the decision and can continue to operate during that process.
It had received an additional two-month extension in September which expired on Sunday.
Uber has faced resistance from regulators and traditional taxi services in a range of countries after being dogged by controversy for a number of years.
Helen Chapman, Director of Licensing, Regulation and Charging at TfL, said:
“As the regulator of private hire services in London we are required to make a decision today on whether Uber is fit and proper to hold a licence. Safety is our absolute top priority.
“While we recognise Uber has made improvements, it is unacceptable that Uber has allowed passengers to get into minicabs with drivers who are potentially unlicensed and uninsured.”
However, Uber said the decision was “extraordinary and wrong” through Jamie Heywood, regional general manager for Northern & Eastern Europe at Uber.
“We have fundamentally changed our business over the last two years and are setting the standard on safety,”
“TfL found us to be a fit and proper operator just two months ago, and we continue to go above and beyond.”
“Over the last two months, we have audited every driver in London and further strengthened our processes.,”
There are around 45,000 Uber drivers in London.